Cytotoxicity of Triterpene Seco-Acids from Betula pubescens Buds

Łukasz Szoka , Walerij Isidorow , Jolanta Nazaruk , Marcin Stocki , Leszek Siergiejczyk

Abstract

The present study investigated the magnitude and mechanism of the cytotoxic effect on selected cancer cell lines of 3,4-seco-urs-4(23),20(30)-dien-3-oic acid (1), 3,4-seco-olean-4(24)-en-19-oxo-3-oic acid (2), and 3,4-seco-urs-4(23),20(30)-dien-19-ol-3-oic acid (3) isolated from downy birch (Betula pubescens) buds by carbon dioxide supercritical fluid extraction and gradient column chromatography. Cell viability in six human cancer lines exposed to these compounds was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was quantified by annexin V/propidium iodide staining of gastric cancer AGS and colorectal cancer DLD-1 cells. To evaluate the mechanism of apoptosis, the expression of apoptosis-related proteins was analyzed by Western blot. Compound 1 exhibited non-specific toxicity, while compounds 2 and 3 were specifically toxic to colon and stomach cancer cells. The toxicity of compounds 2 and 3 against these two cell lines was greater than for compound 1. Cleavage of caspase-8, -9, and -3 was found in AGS and DLD-1 cells treated with all three seco-acids, indicating the induction of apoptosis via extrinsic and intrinsic pathways. Therefore, triterpene seco-acids (1–3) decreased cell viability by apoptosis induction. AGS and DLD-1 cells were more susceptible to seco-acids with an oxidized C19 than normal fibroblasts. Hence, it made them a new group of triterpenes with potential anticancer activity
Author Łukasz Szoka
Łukasz Szoka,,
-
, Walerij Isidorow (FCEE/IIFS)
Walerij Isidorow,,
- Bialystok University of Technology Faculty of Forestry, Hajnówka Campus
, Jolanta Nazaruk
Jolanta Nazaruk,,
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, Marcin Stocki (FCEE/IIFS)
Marcin Stocki,,
- Bialystok University of Technology Faculty of Forestry, Hajnówka Campus
, Leszek Siergiejczyk
Leszek Siergiejczyk,,
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Journal seriesMolecules, ISSN 1420-3049, (N/A 100 pkt)
Issue year2019
Vol24
Pages1-10
Publication size in sheets0.50
Keywords in Englishbirch buds; triterpene seco-acids; cytotoxicity; apoptosis
ASJC Classification Organic Chemistry1605
DOIDOI:10.3390/molecules24224060
URL https://www.mdpi.com/1420-3049/24/22/4060
Internal identifierROC 19-20
Languageen angielski
LicenseUznanie Autorstwa (CC-BY)
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 12-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.146; WoS Impact Factor: 2018 = 3.060 (2) - 2018=3.38 (5)
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