Influence of the Surface Roughness of PEEK GRF30 and Ti6Al4V SLM on the Viability of Primary Human Osteoblasts Determined by the MTT Test

Piotr Prochor , Żaneta Anna Mierzejewska

Abstract

The aim of the study was to clearly determine whether selected modern medical materials and three dimensional printing allow for satisfactory viability of human osteoblasts, which is important from the point of view of the subsequent osseointegration process. Moreover, as implants are produced with various topography, the influence of surface roughness on viability of bone cells was evaluated. To conduct the research, primary human osteoblasts (PromoCell) were used. Cells were seeded on samples of glass-reinforced polyetheretherketone (30% of the filling), Ti6Al4V manufactured with the use of selective laser melting technology and forged Ti6Al4V with appropriately prepared variable surface roughness. To assess the viability of the tested cells the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used. Results showed that all evaluated materials do not exhibit cytotoxic properties. Moreover, on their basis it can be concluded that there is a certain surface topography (designated i.a. as roughness equal to approx. Ra = 0.30 μm), which ensures the highest possible viability of human osteoblasts. On the basis of the received data, it can also be concluded that modern glass-reinforced polyetheretherketone or Ti6Al4V produced by rapid prototyping method allow to manufacture implants that should be effectively used in clinical conditions.
Author Piotr Prochor (FME/IBE)
Piotr Prochor,,
- Department of Biocybernetics and Biomedical Engineering
, Żaneta Anna Mierzejewska (FME/IBE)
Żaneta Anna Mierzejewska,,
- Department of Biocybernetics and Biomedical Engineering
Journal seriesMaterials, ISSN 1996-1944, (N/A 140 pkt)
Issue year2019
Vol12
No24
Pages1-14
Publication size in sheets0.65
Keywords in Englishbiomedical material; cytotoxicity; osteoblasts; sinister laser melting; surface roughness
ASJC Classification General Materials Science2500
DOIDOI:10.3390/ma12244189
URL https://www.mdpi.com/1996-1944/12/24/4189
Internal identifierROC 19-20
Languageen angielski
LicenseUznanie Autorstwa (CC-BY)
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 12-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.285; WoS Impact Factor: 2018 = 2.972 (2) - 2018=3.532 (5)
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