Phytochemistry and biological activities of Polemonium caeruleum L

Grażyna Łaska , Elwira Sieniawska , Łukasz Świątek , Jordan Zjawiony , Shabana Khan , Anastazja Boguszewska , Marcin Stocki , Mirosław Angielczyk , Małgorzata Polz-Dacewicz

Abstract

Polemonium caeruleum L. is a Chinese medicinal plant used in the treatment of tuberculosis, whooping cough, fever, infertility, epilepsy, endometritis and insomnia. It was also used as sedative and anaesthetic agent. In this work the phytochemical investigation using LC-ESI-QTOF-MS and GC–MS techniques was done for chemical characterization of methanol extracts of underground and aerial plant parts. In the course of our studies on medicinal plants, we evaluated the extracts of P. caeruleum for their biological activities as antimicrobial, antimalarial, anti-leishmanial and antitrypanosomal agents and we investigated their cytotoxicity towards mammalian cells – HEK293 and Vero cell lines, both originating from kidney and also against pharyngeal squamous cell carcinoma, FaDu cell line. LC-ESI-QTOF-MS analysis confirmed the presence of triterpene saponins – oleanane derivatives (polemoniumsaponins, glycosides of theasapogenol derivatives and β-amyrin among others), as well as flavonoid glycosides with most predominant acacetin derivatives. Saponins were more abundant and varied in the extract from underground parts of the plant, while flavonoids dominated in the extract from aerial parts. GC–MS performed after silanization enabled the identification of carbohydrates, fatty acid esters, amino acids and carboxylic acids. In the present study, the antibacterial activity of crude extract of the aerial parts of P. caeruleum against Escherichia coli with an IC50 of 137.0 μg/mL and antitrypanosomal activity of crude extract of the underground parts of P. caeruleum with an IC50 of 16.03 μg/mL were reported. The cytotoxicity of the extract from underground parts of P. caeruleum was in a range 44.5–63.5 μg/ml, while cytotoxicity of the extract from aerial parts ranged from 93.8 to 178.0 μg/ml.
Author Grażyna Łaska (FCEE / DAEEM)
Grażyna Łaska,,
- Department of Agri-Food Engineering and Environmental Management
, Elwira Sieniawska
Elwira Sieniawska,,
-
, Łukasz Świątek
Łukasz Świątek,,
-
, Jordan Zjawiony
Jordan Zjawiony,,
-
, Shabana Khan
Shabana Khan,,
-
, Anastazja Boguszewska
Anastazja Boguszewska,,
-
, Marcin Stocki (FF)
Marcin Stocki,,
- Bialystok University of Technology Faculty of Forestry, Hajnówka Campus
, Mirosław Angielczyk
Mirosław Angielczyk,,
-
, Małgorzata Polz-Dacewicz
Małgorzata Polz-Dacewicz,,
-
Journal seriesPhytochemistry Letters, ISSN 1874-3900, (N/A 70 pkt)
Issue year2019
Vol30
Pages314-323
Publication size in sheets0.5
Keywords in EnglishPolemoniaceae, Polemonium caeruleum, Antitrypanosomal activity, Antibacterial activity, Cytotoxicity, Saponins, Flavonoids, LC-ESI-QTOF-MS, GC–MS
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science; 1303 Biochemistry; 1305 Biotechnology
DOIDOI:10.1016/j.phytol.2019.02.017
Internal identifier000044960
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 12-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.922; WoS Impact Factor: 2018 = 1.338 (2) - 2018=1.506 (5)
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