Phytochemical screening of Pulsatilla species and investigation of their biological activities

Grażyna Łaska , Aneta Sienkiewicz , Marcin Stocki , Jordan K. Zjawiony , Vimal Sharma , Andrzej Bajguz , Alicja Piotrowska-Niczyporuk , Melissa Jacob , Shabana Khan

Abstract

The present study aimed to identify biologically active secondary metabolites from the rare plant species, Pulsatilla patens subsp. patens and the cultivated P. vulgaris subsp. vulgaris. Chromatographic fractionation of the ethanolic extract of the roots of P. patens subsp. patens resulted in the isolation of two oleanane-type glycosides identified as hederagenin 3-O-β-d-glucopyranoside (2.7 mg) and hederagenin 3-O-β-d-galactopyranosyl-(1→2)-β-d-glucopyranoside (3.3 mg, patensin). HPLC analysis of the methanolic extract of the crude root of P. patens subsp. patens and P. vulgaris subsp. vulgaris revealed the presence of Pulsatilla saponin D (hederagenin 3-O-α-l-rhamnopyranosyl(1→2)-[β-d-glucopyranosyl(1→4)]-α-l-arabinopyranoside). Chromatographic analysis using GC-MS of the silylated methanolic extracts from the leaves and roots of these species identified the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids. The extracts from Pulsatilla species were tested for their antifungal, antimicrobial, and antimalarial activities, and cytotoxicity to mammalian cell lines. Both P. patens subsp. patens and P. vulgaris subsp. vulgaris were active against the fungus Candida glabrata with the half-maximal inhibitory concentration (IC50) values of 9.37 µg/mL and 11 µg/mL, respectively. The IC50 values for cytotoxicity evaluation were in the range of 32–38 μg/mL for P. patens subsp. patens and 35–57 μg/mL for P. vulgaris subsp. vulgaris for each cell line, indicating general cytotoxic activity throughout the panel of evaluated cancer and noncancer cells.
Author Grażyna Łaska (FCEE / DAEEM)
Grażyna Łaska,,
- Department of Agri-Food Engineering and Environmental Management
, Aneta Sienkiewicz (FCEE / DAEEM)
Aneta Sienkiewicz,,
- Department of Agri-Food Engineering and Environmental Management
, Marcin Stocki (FF)
Marcin Stocki,,
- Bialystok University of Technology Faculty of Forestry, Hajnówka Campus
, Jordan K. Zjawiony
Jordan K. Zjawiony,,
-
, Vimal Sharma
Vimal Sharma,,
-
, Andrzej Bajguz
Andrzej Bajguz,,
-
, Alicja Piotrowska-Niczyporuk
Alicja Piotrowska-Niczyporuk,,
-
, Melissa Jacob
Melissa Jacob,,
-
, Shabana Khan
Shabana Khan,,
-
Journal seriesActa Societatis Botanicorum Poloniae, ISSN 0001-6977, (N/A 40 pkt)
Issue year2019
Vol88
No1
Pages1-17
Publication size in sheets0.8
Keywords in Englishpasque flower subspecies; oleanane-type triterpenoid glycosides; carboxylic acids; antifungal and antimicrobial activities, cytotoxicity, medicinal applications
ASJC Classification1110 Plant Science
DOIDOI:10.5586/asbp.3613
URL https://pbsociety.org.pl/journals/index.php/asbp/article/view/asbp.3613
Internal identifier000045275
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)40
Score sourcejournalList
ScoreBUT score = 30.0, 31-05-2019, manual
Ministerial score = 40.0, 28-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.509; WoS Impact Factor: 2018 = 0.718 (2) - 2018=1.041 (5)
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