Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles
Robert Bucki , Katarzyna Niemirowicz-Laskowska , Piotr Deptuła , Agnieszka Wilczewska , Paweł Misiak , Bonita Durnaś , Krzysztof Fiedoruk , Ewelina Piktel , Joanna Mystkowska , Paul Janmey
AbstractBackground: Magnetic nanoparticles (MNPs) are characterized by unique physicochemical and biological properties that allow their employment as highly biocompatible drug carriers. Gelsolin (GSN) is a multifunctional actin-binding protein involved in cytoskeleton remodeling and free circulating actin sequestering. It was reported that a gelsolin derived phosphoinositide binding domain GSN 160–169, (PBP10 peptide) coupled with rhodamine B, exerts strong bactericidal activity. Results: In this study, we synthesized a new antibacterial and antifungal nanosystem composed of MNPs and a PBP10 peptide attached to the surface. The physicochemical properties of these nanosystems were analyzed by spec troscopy, calorimetry, electron microscopy, and X-ray studies. Using luminescence based techniques and a standard killing assay against representative strains of Gram-positive (Staphylococcus aureus MRSA Xen 30) and Gram-negative (Pseudomonas aeruginosa Xen 5) bacteria and against fungal cells (Candida spp.) we demonstrated that magnetic nanoparticles signifcantly enhance the efect of PBP10 peptides through a membrane-based mode of action, involv ing attachment and interaction with cell wall components, disruption of microbial membrane and increased uptake of peptide. Our results also indicate that treatment of both planktonic and bioflm forms of pathogens by PBP10based nanosystems is more efective than therapy with either of these agents alone. Conclusions: The results show that magnetic nanoparticles enhance the antimicrobial activity of the phosphoi nositide-binding domain of gelsolin, modulate its mode of action and strengthen the idea of its employment for developing the new treatment methods of infections.
|Journal series||Journal of Nanobiotechnology, ISSN 1477-3155, (N/A 140 pkt)|
|Publication size in sheets||0.7|
|Keywords in English||gelsolin, PBP10 peptide, magnetic nanoparticles, fungal cells, antibacterial|
|ASJC Classification||; ; ; ; ;|
|Internal identifier||ROC 19-20|
|License||Journal (articles only); published final; ; with publication|
|Score||= 140.0, 11-03-2020, ArticleFromJournal|
|Publication indicators||: 2018 = 1.21; : 2018 = 5.345 (2) - 2018=5.816 (5)|
|Citation count*||2 (2020-04-02)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.