Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles

Robert Bucki , Katarzyna Niemirowicz-Laskowska , Piotr Deptuła , Agnieszka Wilczewska , Paweł Misiak , Bonita Durnaś , Krzysztof Fiedoruk , Ewelina Piktel , Joanna Mystkowska , Paul Janmey

Abstract

Background: Magnetic nanoparticles (MNPs) are characterized by unique physicochemical and biological properties that allow their employment as highly biocompatible drug carriers. Gelsolin (GSN) is a multifunctional actin-binding protein involved in cytoskeleton remodeling and free circulating actin sequestering. It was reported that a gelsolin derived phosphoinositide binding domain GSN 160–169, (PBP10 peptide) coupled with rhodamine B, exerts strong bactericidal activity. Results: In this study, we synthesized a new antibacterial and antifungal nanosystem composed of MNPs and a PBP10 peptide attached to the surface. The physicochemical properties of these nanosystems were analyzed by spec troscopy, calorimetry, electron microscopy, and X-ray studies. Using luminescence based techniques and a standard killing assay against representative strains of Gram-positive (Staphylococcus aureus MRSA Xen 30) and Gram-negative (Pseudomonas aeruginosa Xen 5) bacteria and against fungal cells (Candida spp.) we demonstrated that magnetic nanoparticles signifcantly enhance the efect of PBP10 peptides through a membrane-based mode of action, involv ing attachment and interaction with cell wall components, disruption of microbial membrane and increased uptake of peptide. Our results also indicate that treatment of both planktonic and bioflm forms of pathogens by PBP10based nanosystems is more efective than therapy with either of these agents alone. Conclusions: The results show that magnetic nanoparticles enhance the antimicrobial activity of the phosphoi nositide-binding domain of gelsolin, modulate its mode of action and strengthen the idea of its employment for developing the new treatment methods of infections.
Author Robert Bucki
Robert Bucki,,
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, Katarzyna Niemirowicz-Laskowska
Katarzyna Niemirowicz-Laskowska,,
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, Piotr Deptuła
Piotr Deptuła,,
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, Agnieszka Wilczewska
Agnieszka Wilczewska,,
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, Paweł Misiak
Paweł Misiak,,
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, Bonita Durnaś
Bonita Durnaś,,
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, Krzysztof Fiedoruk
Krzysztof Fiedoruk,,
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, Ewelina Piktel
Ewelina Piktel,,
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, Joanna Mystkowska (FME / DMPE)
Joanna Mystkowska,,
- Department of Material and Production Engineering
, Paul Janmey
Paul Janmey,,
-
Journal seriesJournal of Nanobiotechnology, ISSN 1477-3155, (N/A 140 pkt)
Issue year2019
Vol17
No81
Pages1-15
Publication size in sheets0.7
Keywords in Englishgelsolin, PBP10 peptide, magnetic nanoparticles, fungal cells, antibacterial
ASJC Classification2701 Medicine (miscellaneous); 1313 Molecular Medicine; 2402 Applied Microbiology and Biotechnology; 3003 Pharmaceutical Science; 1502 Bioengineering; 2204 Biomedical Engineering
DOIDOI:10.1186/s12951-019-0511-1
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6615188/
Internal identifierROC 19-20
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); with publication
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 11-03-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.21; WoS Impact Factor: 2018 = 5.345 (2) - 2018=5.816 (5)
Citation count*2 (2020-04-02)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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