Traumatic acid toxicity mechanisms in human breast cancer MCF-7 cells

Agata Jabłońska-Trypuć , R. Krętowski , Elżbieta Wołejko , Urszula Wydro , Andrzej Butarewicz

Abstract

Traumatic acid (TA) – an oxidative derivative of unsaturated fatty acids, belongs to the cytokinins category – a group of plant hormones, which play an important role in growth and development. Previously we demonstrated its positive influence on oxidative stress parameters in normal human fibroblasts, therefore we decided to investigate its activity in cancer cells. MCF-7 breast cancer cell line was chosen as an experimental model because of proved association between the consumption of dietary fat and the incidence of breast cancer. TA cytotoxicity and its effects on MCF-7 cells proliferation, viability, apoptosis/necrosis, thiol group content, lipid peroxidation, reduced/oxidized glutathione (GSH/GSSG) and ROS (reactive oxygen species) content was examined. The results show a significant effect of TA on tested parameters. TA caused a decrease in cells proliferation and viability, GSH/GSSG ratio and thiol group content. It increases caspase 7 activity, membrane lipid peroxidation and ROS content, simultaneously reducing breast cancer cell growth through oxidative stress influence on apoptosis. The present findings reveal that TA exhibits multiple and complex activity in MCF-7 breast cancer cells and it exhibits potential anticancer properties and tumor preventive activity.
Author Agata Jabłońska-Trypuć (FCEE / DCBB)
Agata Jabłońska-Trypuć,,
- Department of Chemistry, Biology and Biotechnology
, R. Krętowski
R. Krętowski,,
-
, Elżbieta Wołejko (FCEE / DCBB)
Elżbieta Wołejko,,
- Department of Chemistry, Biology and Biotechnology
, Urszula Wydro (FCEE / DCBB)
Urszula Wydro,,
- Department of Chemistry, Biology and Biotechnology
, Andrzej Butarewicz (FCEE / DCBB)
Andrzej Butarewicz,,
- Department of Chemistry, Biology and Biotechnology
Journal seriesRegulatory Toxicology and Pharmacology, ISSN 0273-2300, (N/A 70 pkt)
Issue year2019
Vol106
Pages137-146
Publication size in sheets0.5
Keywords in EnglishTraumatic acid; MCF-7, Oxidative stress, Apoptosis, Reactive oxygen species, Glutathione
ASJC Classification3005 Toxicology; 2700 General Medicine
DOIDOI:10.1016/j.yrtph.2019.04.023
URL https://www.sciencedirect.com/science/article/pii/S02732
Internal identifier000045394
Languageen angielski
LicenseJournal (articles only); published final; Uznanie Autorstwa (CC-BY); after publication
Score (nominal)70
Score sourcejournalList
ScoreBUT score = 30.0
Ministerial score = 70.0, 12-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.005; WoS Impact Factor: 2018 = 2.996 (2) - 2018=2.821 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?