Glutathione Metabolism, Mitochondria Activity, and Nitrosative Stress in Patients Treated for Mandible Fractures

Jan Borys , Mateusz Maciejczyk , Bożena Antonowicz , Adam Krętowski , Jarosław Sidun , Emilia Domel , Jan Dąbrowski , Jerzy Ładny , Katarzyna Morawska , Anna Zalewska


The aim of the study was to evaluate the effect of titanium bone fixations on mitochondrial activity, reactive oxygen species (ROS) production, glutathione metabolism, and selected markers of oxidative/nitrosative stress in the periosteum-like tissue of patients treated with mandible fractures. The study group consisted of 30 patients with bilateral fractures of the mandible body eligible for surgical treatment. Our study is the first one that indicates disturbances of mitochondrial activity as well as a higher production of ROS in the periosteum-like tissue covering titanium fixations of the mandible. We also found significantly higher levels of reduced glutathione and enhanced activity of glutathione reductase in the periosteum homogenates of patients in the study group compared to the control group. Levels of nitrosative (S-nitrosothiols, peroxynitrite, nitrotyrosine) and oxidative stress biomarkers (malondialdehyde, protein carbonyls, dityrosine, kynurenine, and N-formylkynurenine) were statistically elevated in periosteum-like tissue covering titanium fixations. Although exposure to titanium fixations induces local antioxidant mechanisms, patients suffer oxidative damage, and in the periosteum-like tissue the phenomenon of metallosis was observed. Titanium implants cause oxidative/nitrosative stress as well as disturbances in mitochondrial activity.
Author Jan Borys
Jan Borys,,
, Mateusz Maciejczyk
Mateusz Maciejczyk,,
, Bożena Antonowicz
Bożena Antonowicz,,
, Adam Krętowski
Adam Krętowski,,
, Jarosław Sidun (FME/IBE)
Jarosław Sidun,,
- Department of Biocybernetics and Biomedical Engineering
, Emilia Domel
Emilia Domel,,
, Jan Dąbrowski (FME/DMPE)
Jan Dąbrowski,,
- Department of Material and Production Engineering
, Jerzy Ładny
Jerzy Ładny,,
, Katarzyna Morawska
Katarzyna Morawska,,
, Anna Zalewska
Anna Zalewska,,
Journal seriesJournal of Clinical Medicine, ISSN 2077-0383, (N/A 140 pkt)
Issue year2019
Publication size in sheets0.60
Keywords in Englishtitanium fixations, oxidative stress, nitrosative stress, metallosis
Internal identifierROC 19-20
Languageen angielski
LicenseUznanie Autorstwa (CC-BY)
Score (nominal)140
Score sourcejournalList
ScoreBUT score = 5.0, 23-07-2019, manual
Ministerial score = 140.0, 14-02-2020, ArticleFromJournal
Publication indicators WoS Impact Factor: 2018 = 5.688 (2)
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